Zeitschrift für Blutforschung und hämatologische Krankheiten

LinkSeq genotyping: Fast, easy molecular antigen typing

 Matthew Frome

Statement of the Problem:
Blood typing using serology can provide an inaccurate answer if a patient is recently transfused, taking oncology therapeutics like Darzalex, or has weak or partial antigens. Antisera are not always available for rare antigens. Chronically transfused patients can avoid alloimmunization by receiving antigen-negative blood units, but
building a complete patient or donor antigen profile using serology is labor-intensive and can take days. Blood genotyping using traditional methods such as SSP and SSO overcome the limitations of serotyping, but require long, labor-intensive workflows and limited resolution. Blood genotyping using Sanger or NGS sequencing technology have high resolution, but are even more time and labor consuming, expensive and require interpretation by subject matter experts.