Zeitschrift für Chromatographieforschung

Principle of Chromatography

Chromatography is nothing but the separation of mixtures. The liquid is immersed in a mobile phase fluid that takes it through a system that contains another material called the stationary phase. The mixture 's various constituents move at different velocities, allowing them to split. The separation is based on differential partitioning of the stationary and mobile processes. Subtle differences in the partition coefficient of a product result in differential retention on the stationary phase and therefore affect the separation. Chromatography may be either preparatory or analytical. Preparative chromatography has the function of separating the components of a mixture for later use, and is therefore a method of purification. Analytical chromatography is normally done with smaller amounts of material and is meant to determine the presence or the relative proportions of analytes in a mixture. The two aren't missing each other.