Molekulares Klonen und genetische Rekombination

Use of single stranded targeting DNA or negative selection does not provide additional enrichment from a GGTA1 promoter trap

Benjamin P Beaton, Jiude Mao, Clifton N Murphy, Melissa S Samuel, Randall S Prather and Kevin D Wells

Use of single stranded targeting DNA or negative selection does not provide additional enrichment from a GGTA1 promoter trap

Although several techniques have been developed to creategene knockouts in pigs, homologous recombination will continue to be required for site-specific genome modifications that are more sophisticated than gene disruption (base changes, domain exchanges, conditional knockouts). The objective of the present paper was to improve the efficiency of homologous recombination in porcine fetal fibroblasts, which would be used to produce gene knockout pigs by somatic cell nuclear transfer